Alma Mater Studiorum University of Bologna 3 articles published in JoVE Biology Basic Three-Dimensional (3D) Intestinal Model System with an Immune Component Francesca Truzzi1, Silvia Dilloo1, Xinyue Chang1, Anne Whittaker1, Eros D'Amen1, Giovanni Dinelli1 1Department of Agricultural and Food Sciences, Alma Mater Studiorum-University of Bologna Here we describe constructing a basic three-dimensional (3D) intestinal cell line model system and a paraffin embedding protocol for light microscopic evaluation of fixed intestinal equivalents. Staining of selected proteins permits the analysis of multiple visual parameters from a single experiment for potential use in preclinical drug screening studies. Editorial Research Methodologies In Vascular Disease Silvia Fittipaldi1, Francesco Vasuri2 1Department of Experimental Diagnostic and Specialty Medicine (DIMES), Alma Mater Studiorum - University of Bologna, 2Pathology Unit, IRCCS University Hospital of Bologna Medicine Step-by-Step Stapedotomy through Transcanal Exclusive Endoscopic Approach Marella Reale1, Ignacio Javier Fernandez2, Livio Presutti2, Giulia Molinari2 1Department of Otolaryngology-Head and Neck Surgery, University Hospital of Modena, 2Department of Otorhinolaryngology and Audiology, IRCCS Azienda Ospedaliero Universitaria di Bologna; Department of Specialist, Diagnostic and Experimental Medicine, Alma Mater Studiorum University The aim of this article is to provide a step-by-step method for endoscopic stapes surgery from operating room setting and patient positioning to post-operative care. This work would represent a guide for any otologic surgeon who is willing to treat otosclerosis with endoscopic transcanal technique.
