3 articles published in JoVE
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay Mahnoush Bahjat1, Timon A. Bloedjes1, Amélie van der Veen1, Guus de Wilde1, Chiel Maas1, Jeroen E. J. Guikema1 1Department of Pathology, Academic Medical Center, University of Amsterdam, Lymphoma and Myeloma Center Amsterdam (LYMMCARE) Here, it is demonstrated how the in situ Proximity Ligation Assay (PLA) can be used to detect and visualize the direct protein-protein interactions between ATM and p53 in suspension cell cultures exposed to genotoxic stress.
Measuring Cardiac Autonomic Nervous System (ANS) Activity in Children Aimée E. van Dijk*1,2, René van Lien*3,4, Manon van Eijsden2,5, Reinoud J. B. J. Gemke6, Tanja G. M. Vrijkotte1, Eco J. de Geus3,4 1Department of Public Health, Academic Medical Center - University of Amsterdam, 2Department of Epidemiology, Documentation and Health Promotion, Public Health Service of Amsterdam (GGD), 3Department of Biological Psychology, VU University, 4EMGO+ Institute, VU University Medical Center, 5Institute of Health Sciences, VU University, 6Department of Pediatrics, VU University Medical Center Measurement of autonomic nervous system activity usually confines the researcher and participant to the laboratory, which may provide an intimidating environment to children. The VU University Ambulatory Monitoring System (VU-AMS) device can record cardiac autonomic control in any setting. The VU-AMS proved very amenable to testing in children.
The Use of Pharmacological-challenge fMRI in Pre-clinical Research: Application to the 5-HT System Anne Klomp1, Jordi L. Tremoleda2, Anouk Schrantee1, Willy Gsell2, Liesbeth Reneman1 1Department of Radiology, Brain Imaging Center, Academic Medical Center Amsterdam, 2Biological Imaging Centre, MRC Clinical Sciences Centre, Imperial College London The goal of this technique is to assess serotonin (5-HT) neurotransmitter function in the live and free-breathing animal with pharmacological magnetic resonance imaging (phMRI) and an intravenous challenge with a selective serotonin reuptake inhibitor (SSRI), fluoxetine.