3 articles published in JoVE
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets Tanja Vajen1, Alexandra C.A. Heinzmann1, Annemiek Dickhout1, Zhen Zhao2, Magdolna Nagy1, Johan W.M. Heemskerk1, Rory R. Koenen1 1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, 2Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine Leukocytes avidly interact with vascular cells and platelets after vessel wall injury or during inflammation. Here, we describe a straightforward laminar flow-based assay to characterize the molecular mechanisms that underlie the interactions between leukocytes and their cellular partners.
Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells Gisela Nogales-Gadea*1,2, Abhishek Saxena*1, Carolin Hoffmann*1, Judith Hounjet1, Daniëlle Coenen1, Peter Molenaar1, Mario Losen1, Pilar Martinez-Martinez1 1School for Mental Health and Neuroscience, Maastricht University, 2Department of Neurosciences, Institut d'Investigació Germans Trias i Pujol Synthesis of human monoclonal antibodies is the first step in studies aimed at unraveling the pathophysiological mechanisms of auto-antibody-mediated immune responses. We have developed a protocol to generate recombinant human immunoglobulin G (IgG) monoclonal antibodies from blood sorted B cells, including B-cell isolation, antibody cloning and in vitro synthesis.
One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures Wendy L.J. Hansen1, Judith Beuving1, Annelies Verbon2, Petra. F.G. Wolffs1 1Department of Medical Microbiology, Maastricht University Medical Center, 2Department of Internal Medicine, Erasmus Medical Center The design of a straightforward one-day workflow scheme for bacterial pathogen diagnostics enables the rapid recognition of bloodstream infections. The inclusion of eight clinically relevant bacterial targets and their antibiotic resistance profiles offers the clinician an initial insight on the same day, which can lead to more adequate therapy.