Skip to content
5 articles published in JoVE
-
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
Markus Götz1, Philipp Wortmann1, Sonja Schmid1,2, Thorsten Hugel1
1Institute of Physical Chemistry, University of Freiburg, 2Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology
Here, we present a protocol to obtain three-color smFRET data and its analysis with a 3D ensemble Hidden Markov Model. With this approach, scientists can extract kinetic information from complex protein systems, including cooperativity or correlated interactions.
-
Laser-heating and Radiance Spectrometry for the Study of Nuclear Materials in Conditions Simulating a Nuclear Power Plant Accident
Dario Manara1, Luca Soldi1,2,4, Sara Mastromarino1,3,5, Kostantinos Boboridis1, Davide Robba1, Luka Vlahovic1, Rudy Konings1
1European Commission, Joint Research Centre, 2Energy Department, Politecnico di Milano, 3Department of Chemical Physics, Sapienza - Università di Roma, 4CEA Saclay, 5TU Delft
We present experiments in which real nuclear fuel, cladding, and containment materials are laser heated to temperatures beyond 3,000 K while their behavior is studied by radiance spectroscopy and thermal analysis. These experiments simulate, on a laboratory scale, the formation of a lava-phase following a nuclear reactor core meltdown.
-
An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage
Vahid Arbabi*1,2, Behdad Pouran*1,2, Amir. A. Zadpoor1, Harrie Weinans1,2,3
1Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology (TU Delft), 2Department of Orthopedics, UMC Utrecht, 3Department of Rheumatology, UMC Utrecht
We propose a protocol to investigate the transport of charged and uncharged molecules across articular cartilage with the aid of recently developed experimental and numerical methods.
-
-
Get cutting-edge science videos from JoVE sent straight to your inbox every month.