3 articles published in JoVE
An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage Vahid Arbabi*1,2, Behdad Pouran*1,2, Amir. A. Zadpoor1, Harrie Weinans1,2,3 1Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology (TU Delft), 2Department of Orthopedics, UMC Utrecht, 3Department of Rheumatology, UMC Utrecht We propose a protocol to investigate the transport of charged and uncharged molecules across articular cartilage with the aid of recently developed experimental and numerical methods.
Application of an In vitro DNA Protection Assay to Visualize Stress Mediation Properties of the Dps Protein Vlad O. Karas1, Ilja Westerlaken1, Anne S. Meyer1 1Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology The DNA-binding protein from starved cells (Dps) plays a crucial role in combating bacterial stress. This article discusses the purification of E. coli Dps and the protocol for an in vitro assay demonstrating Dps-mediated protection of DNA from degradation by reactive oxygen species.
A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments Eva K. Brinkman1, Kira Schipper1, Nadine Bongaerts1, Mathias J. Voges1, Alessandro Abate2, S. Aljoscha Wahl1 1Department of Biotechnology, Delft University of Technology, 2Delft Center for Systems and Control, Delft University of Technology A sustainable auto regulating bacterial system for the remediation of oil pollutions was designed using standard interchangeable DNA parts (BioBricks). An engineered E. coli strain was used to degrade alkanes via β-oxidation in toxic aqueous environments. The respective enzymes from different species showed alkane degradation activity. Additionally, an increased tolerance to n-hexane was achieved by introducing genes from alkane-tolerant bacteria.