2 articles published in JoVE
Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry Suzan Wetzels*1,2,3, Mitchell Bijnen*1,2, Erwin Wijnands2,4, Erik A.L. Biessen2,4, Casper G. Schalkwijk1,2, Kristiaan Wouters1,2 1Department of Internal Medicine, MUMC, 2CARIM, MUMC, 3Department of Immunology and Biochemistry, Biomedical Research Institute, Hasselt University, 4Department of Pathology, MUMC This article describes a method to analyze immune cell content of adipose tissue by isolation of immune cells from adipose tissue and subsequent analysis using flow cytometry.
Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair Jian Li1, Holger Jahr2,3, Wei Zheng4, Pei-Gen Ren1 1Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 2Department of Orthopedic Surgery, Maastricht UMC+, 3Department of Orthopaedic Surgery, University Hospital RWTH, 4Research Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences Here, we present a protocol to visualize blood vessel formation in vivo and in real-time in 3D scaffolds by multiphoton microscopy. Angiogenesis in genetically modified scaffolds was studied in a murine calvarial critical bone defect model. More new blood vessels were detected in the treatment group than in controls.