2 articles published in JoVE
A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells Daniëlle G. Leuning1, Ellen Lievers1, Marlies E.J. Reinders1, Cees van Kooten1, Marten A. Engelse1, Ton J. Rabelink1 1Department of Internal Medicine, Leiden University Medical Centre Here we present a novel clinical grade isolation and culture method for kidney Perivascular Stromal Cells (kPSCs) based on whole organ perfusion with digestive enzymes and NG2-cell enrichment. With this method, it is possible to acquire sufficient cell numbers for cellular therapy.
Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples Simone Punt1, Robert J. Baatenburg de Jong2, Ekaterina S. Jordanova1,3 1Department of Pathology, Leiden University Medical Center, 2Department of Otorhinolaryngology and Head and Neck Surgery, Erasmus University Medical Center, 3Center for Gynecological Oncology Amsterdam, VU Medical Center Multiparameter fluorescence immunohistochemistry can be used to assess the number, relative distribution, and localization of immune cell populations in the tumor microenvironment. This manuscript describes the use of this technique to analyze T-cell subpopulations in oropharyngeal cancer.