3 articles published in JoVE
Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification Dwayne B. Holmes1, Vivi M. Heine1,2 1Department of Pediatrics/Child Neurology, Amsterdam Neuroscience, VU University Medical Center, 2Department of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam We describe a simplified 3D differentiation protocol for hPSCs, using defined medium and reduced growth factors, capable of generating cell aggregates with early neuroepithelial structures and positive for cerebellar-associated markers, as well as an optional 2D modification for differentiating cells as a monolayer to generate functional neurons.
Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods Nieke Westerik1,2, Alex Paul Wacoo1,2,3, Wilbert Sybesma1, Remco Kort1,2,4,5 1Yoba for Life Foundation, 2Department of Molecular Cell Biology, Vrije Universiteit Amsterdam (VUA), 3Uganda Industrial Research Institute (UIRI), 4Micropia, Natura Artis Magistra, 5Department of Microbiology and Systems Biology, Netherlands Organisation for Applied Scientific Research (TNO) A production protocol is described for the small-scale production of probiotic fermented dairy drink with the aid of a novel bacterial starter culture.
Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus Mark Slabodnick1,2, Bram Prevo1,3, Peter Gross1,4, Janet Sheung1,5, Wallace Marshall1,2 1Physiology Course, Marine Biological Laboratory, 2Department of Biochemistry & Biophysics, University of California San Francisco, 3Department of Physics and Astronomy, Vrije Universiteit Amsterdam, 4Max Planck Institute of Molecular Cell Biology and Genetics, 5Department of Physics, University of Illinois Urbana-Champaign The giant ciliate Stentor coeruleus is a classical system for studying regeneration and wound healing in single cells. By imaging Stentor cells simultaneously at low and high magnification it is possible to measure cytoplasmic flows before, during, and after wounding.