Sanquin research and Landsteiner laboratory 1 article published in JoVE Neuroscience Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia Donata De Marco1, Renske Taggenbrock2,3, Rosa Crespo3, Wouter Koudstaal3, Elizabeth Ramsburg4, Adrian Apetri3 1Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Belgium, 2Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, The Netherlands, 3Janssen Prevention Center, Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands, 4Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania Here we describe a cell-based assay to quantitatively assess tau uptake by microglia with the aim of creating an investigational tool to better characterize the mechanisms of action of anti-tau antibodies.
