Hubrecht Institute-KNAW Utrecht 1 article published in JoVE Biology Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids Ravian L. van Ineveld*1,2,3, Hendrikus C.R. Ariese*1,2,3, Ellen J. Wehrens1,2,3, Johanna F. Dekkers*1,2,3,4, Anne C. Rios*1,2,3 1Princess Máxima Center for Pediatric Oncology, 2Department of Cancer Research, Oncode Institute, Hubrecht Institute-KNAW Utrecht, 3Cancer Genomics Center (CGC), 4Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht The entire 3D structure and cellular content of organoids, as well as their phenotypic resemblance to the original tissue can be captured using the single-cell resolution 3D imaging protocol described here. This protocol can be applied to a wide range of organoids varying in origin, size and shape.
