Radboud Institute for Molecular Life Sciences 1 article published in JoVE Genetics Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis Jos G.A. Smits*1, Jieqiong Qu*1, Hanna Niehues2, Huiqing Zhou1,3 1Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands, 2Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands, 3Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands Presented here is a stepwise procedure for in vitro differentiation of human primary keratinocytes by contact inhibition followed by characterization at the molecular level by RNA-seq analysis.
