3 articles published in JoVE
Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance Marinke W. van der Helm1, Mathieu Odijk1, Jean-Philippe Frimat1,2, Andries D. van der Meer3, Jan C.T. Eijkel1, Albert van den Berg1, Loes I. Segerink1 1BIOS Lab on a Chip group, MIRA Institute for Biomedical Technology and Technical Medicine, MESA+ Institute for Nanotechnology and Max Planck Center for Complex Fluid Dynamics, University of Twente, 2Microsystems, Eindhoven University of Technology, 3Applied Stem Cell Technologies, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente This publication describes the fabrication of an organ-on-chip device with integrated electrodes for direct quantification of transendothelial electrical resistance (TEER). For validation, the blood-brain barrier was mimicked inside this microfluidic device and its barrier function was monitored. The presented methods for electrode integration and direct TEER quantification are generally applicable.
3D Whole-heart Myocardial Tissue Analysis Hans Thijs Van den Broek1, Leon De Jong1,2, Pieter A. Doevendans1, Steven A.J. Chamuleau1, Frebus J. Van Slochteren*1, René Van Es*1 1Department of Cardiology, Division Heart and Lungs, University Medical Center Utrecht, 2MIRA Institute, University Twente This protocol describes a novel method for the 3D comparison of whole-heart myocardial tissue with MRI. This is designed for the accurate assessment of intramyocardial injections in the infarct border zone of a chronic porcine model of myocardial infarction.
Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation Stefan Giselbrecht1, Eric Gottwald1, Roman Truckenmueller2, Christina Trautmann3, Alexander Welle1, Andreas Guber4, Volker Saile4, Thomas Gietzelt5, Karl-Friedrich Weibezahn1 1Institute for Biological Interfaces, Karlsruhe Research Centre, 2Institute for BioMedical Technology, University of Twente, 3Department of Materials Research, Institute for Heavy Ion Research, 4Institute of Microstructure Technology, Karlsruhe Research Centre, 5Institute for Micro Process Engineering, Karlsruhe Research Centre We present two processes for the microfabrication of porous polymer chips for three-dimensional cell cultivation. The first one is hot embossing combined with a solvent vapour welding process. The second one uses a recently developed microthermoforming process combined with ion track technology leading to a significant simplification of manufacture.