2 articles published in JoVE
3D Whole-heart Myocardial Tissue Analysis Hans Thijs Van den Broek1, Leon De Jong1,2, Pieter A. Doevendans1, Steven A.J. Chamuleau1, Frebus J. Van Slochteren*1, René Van Es*1 1Department of Cardiology, Division Heart and Lungs, University Medical Center Utrecht, 2MIRA Institute, University Twente This protocol describes a novel method for the 3D comparison of whole-heart myocardial tissue with MRI. This is designed for the accurate assessment of intramyocardial injections in the infarct border zone of a chronic porcine model of myocardial infarction.
Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation Stefan Giselbrecht1, Eric Gottwald1, Roman Truckenmueller2, Christina Trautmann3, Alexander Welle1, Andreas Guber4, Volker Saile4, Thomas Gietzelt5, Karl-Friedrich Weibezahn1 1Institute for Biological Interfaces, Karlsruhe Research Centre, 2Institute for BioMedical Technology, University of Twente, 3Department of Materials Research, Institute for Heavy Ion Research, 4Institute of Microstructure Technology, Karlsruhe Research Centre, 5Institute for Micro Process Engineering, Karlsruhe Research Centre We present two processes for the microfabrication of porous polymer chips for three-dimensional cell cultivation. The first one is hot embossing combined with a solvent vapour welding process. The second one uses a recently developed microthermoforming process combined with ion track technology leading to a significant simplification of manufacture.