2 articles published in JoVE
High-pressure, High-temperature Deformation Experiment Using the New Generation Griggs-type Apparatus Jacques Précigout1, Holger Stünitz1,2, Yves Pinquier3, Rémi Champallier1, Alexandre Schubnel3 1Institut des Sciences de la Terre d’Orléans (ISTO), UMR 7327, CNRS-BRGM, Université d’Orléans, 2Department of Geology, University of Tromsø, 3Laboratoire de Géologie, UMR 8538, CNRS, Ecole Normale Supérieure (ENS Paris) Rock deformation needs to be quantified at high pressure. A description of the procedure to perform deformation experiments in a newly designed solid-medium Griggs-type apparatus is here given. This provides technological basis for future rheological studies at pressures up to 5 GPa.
Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy Chun A. Changou1,2, Deanna L. Wolfson2, Balpreet Singh Ahluwalia2,3, Richard J. Bold4,5, Hsing-Jien Kung5,6, Frank Y.S. Chuang1,2,5 1Department of Biochemistry and Molecular Medicine, University of California, Davis, 2NSF Center for Biophotonics Science & Technology, University of California, Davis, 3University of Tromsø, 4Department of Surgery (Division of Surgical Oncology), University of California, Davis, 5UC Davis Comprehensive Cancer Center, University of California, Davis, 6Department of Biological Chemistry, University of California, Davis Autophagy is a ubiquitous process that enables cells to degrade and recycle proteins and organelles. We apply advanced fluorescence microscopy to visualize and quantify the small, but essential, physical changes associated with the induction of autophagy, including the formation and distribution of autophagosomes and lysosomes, and their fusion into autolysosomes.