2 articles published in JoVE
Tickling, a Technique for Inducing Positive Affect When Handling Rats Sylvie Cloutier1,2, Megan R. LaFollette3, Brianna N. Gaskill3, Jaak Panksepp1, Ruth C. Newberry4 1Center for the Study of Animal Well-being, Department of Integrative Physiology and Neuroscience, Washington State University, 2Canadian Council on Animal Care, 3Department of Animal Sciences, Center for Animal Welfare Science, College of Agriculture, Purdue University, 4Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences This article demonstrates the standardized application of playful handling, a tickling technique designed to mimic rat rough-and-tumble play. This technique is effective at reducing fearful reactions to humans and generating positive affect when rats are handled for common husbandry activities and medical and research procedures such as injection.
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice Chrstine Bäuerl*1, Özgun C.O. Umu*2, Pablo E. Hernandez3, Dzung B. Diep4, Gaspar Pérez-Martínez1 1Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), 2Department of Food Safety and Infection Biology, Norwegian University of Life Sciences (NMBU), 3Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid (UCM), 4Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU) Bacteriocins are believed to play a key role in defining microbial diversity in different ecological niches. Here, we describe an efficient procedure to assess how bacteriocins affect gut microbiota composition in an animal model.