University of Wroclaw 3 articles published in JoVE Biochemistry Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells Sebastien Mailfert*1, Karolina Wojtowicz*2, Sophie Brustlein*1, Ewa Blaszczak3, Nicolas Bertaux4, Marcin Łukaszewicz2, Didier Marguet1, Tomasz Trombik2 1CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, 2Faculty of Biotechnology, University of Wroclaw, 3Faculty of Biological Sciences, University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells. Biology Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay Wojciech Wiertelak1, Mariusz Olczak1, Dorota Maszczak-Seneczko1 1Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw The protocol presented here is intended to demonstrate the occurrence of heterologous interactions between Golgi-resident type III membrane proteins with cytoplasmically exposed N- and/or C-termini in live mammalian cells using the most recent variant of the split luciferase complementation assay. Biology Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy Łukasz Makowski1, Damian Trojanowski1, Jolanta Zakrzewska-Czerwińska1 1Department of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw Presented here is a protocol that describes monitoring of the complete life cycle of predatory bacterium Bdellovibrio bacteriovorus using time-lapse fluorescence microscopy in combination with an agarose pad and cell-imaging dishes.
