University of Lisbon 1 article published in JoVE Developmental Biology Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture Pedro Gameiro dos Santos1, Ana Rita Soares1, Sólveig Thorsteinsdóttir1, Gabriela Rodrigues1 1Centre for Ecology, Evolution and Environmental Changes and Global Change & Sustainability Institute, Department of Animal Biology, Faculty of Sciences, University of Lisbon In this work, a decellularization protocol was optimized to obtain decellularized matrices of fetal mouse skeletal muscle. C2C12 myoblasts can colonize these matrices, proliferate, and differentiate. This in vitro model can be used to study cell behavior in the context of skeletal muscle diseases such as muscular dystrophies.
