1 article published in JoVE
The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression Per R. Jonsson1, Anna-Lisa Wrange3, Ulrika Lind2, Anna Abramova2, Martin Ogemark1, Anders Blomberg2 1Department of Marine Sciences, University of Gothenburg, 2Department of Chemistry and Molecular Biology, University of Gothenburg, 3IVL Swedish Environmental Research Institute The barnacle Balanus (Amphibalanus) improvisus is a model for studying osmoregulation and antifouling. However, natural seasonal spawning yields an unpredictable supply of cyprid larvae. Here, a protocol for the all-year-round culturing of B. improvisus is described, including the production of larvae. The use of cultured barnacles in gene expression studies is illustrated.