Wallenberg Centre for Molecular Medicine (WCMM) 1 article published in JoVE Biochemistry Kinetic Screening of Nuclease Activity using Nucleic Acid Probes Alien Balian*1,2,3, Javier Garcia Gonzalez*1,2,3, Nora Bastida1,3, Khadija-Tul Kubra Akhtar1,3, Baris A. Borsa1,2,3, Frank J. Hernandez1,2,3 1Department of Physics, Chemistry and Biology, Linköping University, 2Wallenberg Centre for Molecular Medicine (WCMM), 3Nucleic Acids Technologies Laboratory (NAT-lab), Linköping University Altered nuclease activity has been associated with different human conditions, underlying its potential as a biomarker. The modular and easy to implement screening methodology presented in this paper allows the selection of specific nucleic acid probes for harnessing nuclease activity as a biomarker of disease.
