National Institute of Chemistry 1 article published in JoVE Environment Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis Manca Kovač Viršek1, Andreja Palatinus1, Špela Koren1, Monika Peterlin1, Petra Horvat2, Andrej Kržan2 1Sector for Marine Waters, Institute for water of the Republic of Slovenia, 2Laboratory for Polymer Chemistry and Technology, National Institute for Chemistry Slovenia The protocol below describes the methodology for: microplastics sampling on the sea surface, separation of microplastic and chemical identification of particles. This protocol is in line with the recommendations for microplastics monitoring published by the MSFD Technical Subgroup on Marine Litter.