3 articles published in JoVE
A Versatile Model of Hard Tick Infestation on Laboratory Rabbits Consuelo Almazán1, Sarah Bonnet1, Martine Cote1, Mirko Slovák2, Yoonseong Park3, Ladislav Šimo1 1Unité Mixte de Recherche (UMR) Biologie moléculaire et immunologie parasitaires (BIPAR), Institut national de la recherche agronomique (INRA), Ecole Nationale Vétérinaire d'Alfort, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Paris-Est, 2Institute of Zoology, Slovak Academy of Sciences, 3Department of Entomology, Kansas State University We have developed a simple and versatile system to feed hard ticks on laboratory rabbits. Our non-laborious protocol uses easily accessible materials and can be adjusted depending on the requirements of the various experimental settings. The method allows comfortable monitoring and/or sampling of ticks during the entire feeding period.
Methods of Ex Situ and In Situ Investigations of Structural Transformations: The Case of Crystallization of Metallic Glasses Marcel B. Miglierini1,2, Vít Procházka3, Vlastimil Vrba3, Peter Švec4, Dušan Janičkovič4, Peter Matúš5 1Institute of Nuclear and Physical Engineering, Slovak University of Technology in Bratislava, Slovakia, 2Department of Nuclear Reactors, Czech Technical University in Prague, Czech Republic, 3Department of Experimental Physics, Palacky University Olomouc, Czech Republic, 4Institute of Physics, Slovak Academy of Sciences, Bratislava, Slovakia, 5Institute of Laboratory Research on Geomaterials, Faculty of Natural Sciences, Comenius University in Bratislava, Slovakia Here, we present a protocol to describe ex situ and in situ investigations of structural transformations in metallic glasses. We employed nuclear-based analytical methods which inspect hyperfine interactions. We demonstrate the applicability of Mössbauer spectrometry and nuclear forward scattering of synchrotron radiation during temperature-driven experiments.
Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice Takahiro Tadokoro1, Atsushi Miyanohara1, Michael Navarro1, Kota Kamizato1, Stefan Juhas2, Jana Juhasova2, Silvia Marsala1, Oleksandr Platoshyn1, Erik Curtis3, Brandon Gabel3, Joseph Ciacci3, Nada Lukacova4, Katarina Bimbova4, Martin Marsala1,4 1Neuroregeneration Laboratory, Department of Anesthesiology, University of California, San Diego, 2Institute of Animal Physiology and Genetics, Czech Academy of Sciences, 3Department of Neurosurgery, University of California, San Diego, 4Institute of Neurobiology, Slovak Academy of Sciences The goal of the present study was to develop and validate the potency and safety of spinal adeno-associated virus 9 (AAV9)-mediated gene delivery by using a novel subpial gene delivery technique in adult mice.