4 articles published in JoVE
Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina Iskalen Cansu Topcu Okan1,2, Mehri Ahmadian1,2, Yesim Tutuncu1,2, Halit Yusuf Altay1,2, Cavit Agca1,2 1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Nanotechnology Research and Application Center (SUNUM), Sabanci University This protocol presents how to quantify AAV transduction efficiency in mouse retina using digital droplet PCR (dd-PCR) together with small scale AAV production, intravitreal injection, retinal imaging, and retinal genomic DNA isolation.
A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles Sina Abdolhosseinzadeh1,2, Mirsajjad Mousavi3, Navid Haghmoradi3, Selmiye Alkan Gürsel3,4 1Institut des matériaux, École Polytechnique Fédérale de Lausanne (EPFL), 2Laboratory for Functional Polymers, Swiss Federal Laboratories for Materials Science and Technology (Empa), 3Faculty of Engineering and Natural Sciences, Sabanci University, 4SUNUM Nanotechnology Research Centre, Sabanci University For a continuous and scalable synthesis of noble-metal-based nanocomposites, a novel photocatalytic reactor is developed and its structure, operation principles, and product quality optimization strategies are described.
Diagonal Method to Measure Synergy Among Any Number of Drugs Melike Cokol-Cakmak1, Feray Bakan2, Selim Cetiner1, Murat Cokol1,2,3 1Faculty of Engineering and Natural Sciences, Sabanci University, 2Nanotechnology Research and Application Center, Sabanci University, 3Laboratory of Systems Pharmacology, Harvard Medical School In this protocol, we describe how to make Loewe additivity-based drug interaction measurements for pairwise and three-way drug combinations.
Study of Protein-protein Interactions in Autophagy Research Secil Erbil-Bilir1, Nur M. Kocaturk1, Melih Yayli2, Devrim Gozuacik1,3 1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Information Center, Sabanci University, 3EFSUN, Center of Excellence for Functional Surfaces and Interfaces for Nano Diagnostics, Sabanci University Presented here are two antibody-based protein-protein interaction research techniques: immunofluorescence and immunoprecipitation. These techniques are suitable for studying physical interactions between proteins for the discovery of novel components of cellular signaling pathways and for understanding protein dynamics.