University of Ontario Institute of Technology View Institution's Website 3 articles published in JoVE Biochemistry Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria Richard V. Augimeri1, Andrew J. Varley1, Janice L. Strap1 1Molecular Microbial Biochemistry Laboratory, Faculty of Science (Applied Bioscience Program), University of Ontario Institute of Technology The protocols outlined herein facilitate the convenient investigation of bacterial ethylene responses by utilizing 2-chloroethylphosphonic acid (CEPA). Ethylene is produced in situ through the decomposition of CEPA in an aqueous bacterial growth medium, circumventing the requirement for pure ethylene gas. Chemistry Metal-free Synthesis of Ynones from Acyl Chlorides and Potassium Alkynyltrifluoroborate Salts Cassandra L. Taylor1, Yuri Bolshan1 1Faculty of Science, University of Ontario Institute of Technology The goal of this manuscript is to demonstrate a straightforward method for the preparation of ynones from acyl chloride and potassium alkynyltrifluoroborate salt starting materials. The one-pot reaction proceeds rapidly in the presence of boron trichloride without exclusion of air and moisture. Immunology and Infection Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay Derek Tilley1, Irina Levit2, John A. Samis1,3 1Applied Bioscience Program, Faculty of Science, University of Ontario Institute of Technology, 2Nursing Program, Faculty of Health Sciences, University of Ontario Institute of Technology, 3Medical Laboratory Science Program, Faculty of Health Sciences, University of Ontario Institute of Technology This study describes a novel microplate assay that measures FV coagulation activity during fibrin clot formation in human plasma which has not been reported previously. The method uses a kinetic microplate reader to continuously measure the change in absorbance at 405nm during fibrin clot formation in human plasma.
