Hotel-Dieu de Quebec 2 articles published in JoVE Biochemistry Quantitative Immunofluorescence to Measure Global Localized Translation Jonathan Bergeman1, Marc-Étienne Huot1,2 1Centre de Recherche sur le Cancer de l’Université Laval, Faculté de Médecine, Département de Biologie moléculaire, biochimie médicale et pathologie, Université Laval, 2CRCHU de Québec: L’Hôtel-Dieu de Québec This manuscript describes a method to visualize and quantify localized translation events in subcellular compartments. The approach proposed in this manuscript requires a basic confocal imaging system and reagents and is rapid and cost-effective. Biology GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins Ranjan Maity*1, Joris Pauty*1, Jana Krietsch*1, Rémi Buisson1, Marie-Michelle Genois1, Jean-Yves Masson1 1Genome Stability Laboratory, Oncology Axis, Hôtel-Dieu de Québec In the present protocol, we demonstrate a highly efficient and cost-effective small-scale protein purification method, which allows purification of recombinant proteins by uniquely combining a cleavable GST-tag and a small His-tag.
