British Columbia Children's Hospital & University of British Columbia 2 articles published in JoVE Immunology and Infection Nasal Brushing Sampling and Processing Using Digital High Speed Ciliary Videomicroscopy – Adaptation for the COVID-19 Pandemic Noemie Bricmont1, Lionel Benchimol2, Anne-Lise Poirrier2, Christine Grignet3, Claire Seaton4, Mark A. Chilvers4, Marie-Christine Seghaye5, Renaud Louis1,6, Philippe Lefebvre2, Celine Kempeneers1,7 1Pneumology laboratory, I3 Group, GIGA Research Center, University of Liège, 2Department of ENT, University Hospital of Liège, 3Department for Occupational Safety and Health, Biosafety and Biosecurity Unit, University of Liège, 4 To guarantee a successful and high-quality ciliary functional analysis for PCD diagnosis, a precise and careful method for respiratory epithelium sampling and processing is essential. To continue providing PCD diagnostic service during the COVID-19 pandemic, the ciliary videomicroscopy protocol has been updated to include appropriate infection control measures. Medicine Solubility of Hydrophobic Compounds in Aqueous Solution Using Combinations of Self-assembling Peptide and Amino Acid Shaun Pacheco1, Shan-Yu Fung2, Mingyao Liu1,3 1Latner Thoracic Surgery Research Laboratories, University Health Network, 2 This protocol describes a clinically-applicable means of dissolving hydrophobic compounds in an aqueous environment using combinations of self-assembling peptide and amino acid solutions. Our method resolves a major limitation of hydrophobic therapeutics, which lack safe, efficient means of solubility and delivery methods into clinical settings.
