Peter Munk Cardiac Centre 1 article published in JoVE Immunology and Infection Isolation and Identification of Extravascular Immune Cells of the Heart Laura Aronoff1,2, Slava Epelman1,2,3,4,5, Xavier Clemente-Casares1,2 1Toronto General Hospital Research Institute, University Health Network (UHN), 2Dept of Laboratory Medicine and Pathobiology, University of Toronto, 3Dept of Immunology, University of Toronto, 4Peter Munk Cardiac Centre, 5Ted Rogers Centre for Heart Research This protocol presents a simple and efficient method to isolate, identify and quantify immune cells residing in the myocardium of mice during steady state or inflammation. The protocol combines enzymatic and mechanical digestion for the generation of a single cell suspension that can be further analyzed by flow cytometry.
