The Hospital for Sick Children, Toronto 2 articles published in JoVE Biology An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis Yohei Tomita1, Zhuo Shao2, Bertan Cakir1, Yumi Kotoda1, Zhongjie Fu1,3, Lois E.H. Smith1 1Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, 2Department of Clinical and Metabolic Genetics, Hospital for Sick Children, University of Toronto, 3Manton Center for Orphan Disease, Harvard Medical School, Boston Children's Hospital This protocol presents choroid sprouting assay, an ex vivo model of microvascular proliferation. This assay can be used to assess pathways involved in proliferating choroidal micro vessels and assess drug treatments using wild type and genetically modified mouse tissue. Medicine Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation J. Moritz Kaths1,2, Vinzent N. Spetzler1, Nicolas Goldaracena1, Juan Echeverri1, Kristine S. Louis1, Daniel B. Foltys3, Mari Strempel4, Paul Yip5, Rohan John5, Istvan Mucsi1, Anand Ghanekar1, Darius Bagli6,7, Lisa Robinson2, Markus Selzner1 1Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, 2Division of Nephrology, The Hospital for Sick Children, Toronto, 3Department of General, Visceral & Transplant Surgery, University Medical Center Mainz, 4Department of Abdominal, Vascular & Transplant Surgery, Merheim Medical Center Cologne, 5Laboratory Medicine & Pathobiology, Toronto General Hospital, 6Departments of Surgery (Urology) & Physiology, The Hospital for Sick Children, Toronto, 7Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
