Galenvs Sciences, Inc. 1 article published in JoVE Bioengineering Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics Lidija Malic1, Abdelrahman Elmanzalawy1, Jamal Daoud2, Matthias Geissler1, Alex Boutin1, Ljuboje Lukic1, Mojra Janta1, Dillon Da Fonte1, Christina Nassif1, Teodor Veres1 1Life Sciences Division, National Research Council of Canada, 2Galenvs Sciences, Inc. Epigenetic markers are used for white blood cell (WBC) subtyping through the quantification of DNA methylation patterns. This protocol presents a multiplex droplet polymerase chain reaction (mdPCR) method using a thermoplastic elastomer (TPE)-based microfluidic device for droplet generation allowing for precise and multiplex methylation-specific target quantification of WBC differential counts.