Genome Stability Laboratory, CHU de Quebec Research Center, HDQ Pavilion 1 article published in JoVE Biology Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging Julia O'Sullivan*1,2, Sofiane Y. Mersaoui*1,2, Guy Poirier2,3, Jean-Yves Masson1,2 1Oncology Division, Genome Stability Laboratory, CHU de Québec Research Center, HDQ Pavilion, 2Department of Molecular Biology, Medical Biochemistry, and Pathology, Laval University Cancer Research Center, 3Oncology Division, CHU de Québec Research Center, CHUL Pavilion In the present protocol, we demonstrate how to visualize DNA double-strand end resection during S/G2 phase of the cell cycle using an immunofluorescence-based method.
