Canadian Donation and Transplantation Research Program 2 articles published in JoVE Medicine Normothermic Negative Pressure Ventilation Ex Situ Lung Perfusion: Evaluation of Lung Function and Metabolism Keir A. Forgie1,2, Nicholas Fialka3, Max Buchko1,2, Sayed Himmat4, Sanaz Hatami4, Xiao Qi4, Xiuhua Wang4, Katie-Marie Buswell5, Ryan Edgar5, Darren H. Freed1,2,6,7, Jayan Nagendran1,2,6,7 1Division of Cardiac Surgery, Department of Surgery, Faculty of Medicine, University of Alberta, 2Mazankowski Alberta Heart Institute, 3Faculty of Medicine and Dentistry, University of Alberta, 4Department of Surgery, Faculty of Medicine, University of Alberta, 5Ray Rajotte Surgical-Medical Research Institute (SMRI), University of Alberta, 6Alberta Transplant Institute, 7Canadian Donation and Transplantation Research Program This paper describes a porcine model of negative pressure ventilation ex situ lung perfusion, including procurement, attachment, and management on the custom-made platform. Focus is made on anesthetic and surgical techniques, as well as troubleshooting. Medicine Left Lung Orthotopic Transplantation in a Juvenile Porcine Model for ESLP Keir A. Forgie1,2, Nicholas Fialka3, Mubashir Khan4, Max Buchko1,2, Sanaz Hatami4, Sayed Himmat4, Xiao Qi4, Xiuhua Wang4, Katie-Marie Buswell5, Ryan Edgar5, Daina Domahidi5, Darren H. Freed1,2,6,7, Jayan Nagendran1,2,6,7 1Division of Cardiac Surgery, Department of Surgery, Faculty of Medicine, University of Alberta, 2Mazankowski Alberta Heart Institute, 3Faculty of Medicine and Dentistry, University of Alberta, 4Department of Surgery, Faculty of Medicine, University of Alberta, 5Ray Rajotte Surgical-Medical Research Institute (SMRI), University of Alberta, 6Alberta Transplant Institute, 7Canadian Donation and Transplantation Research Program This protocol describes a juvenile porcine model of orthotopic left lung allotransplantation designed for use with ESLP research. Focus is made on anesthetic and surgical techniques, as well as critical steps and troubleshooting.
