Proteoform Scientific Inc. 1 article published in JoVE Chemistry Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry Jessica L. Nickerson1, Venus Baghalabadi1, Ziheng Dang1, Victoria A. Miller2, Sara L. Little2, Alan A. Doucette1 1Department of Chemistry, Dalhousie University, 2Proteoform Scientific Inc. The present protocol describes solvent-based protein precipitation under controlled conditions for robust and rapid recovery and purification of proteome samples prior to mass spectrometry.