Sinai Health Systems 1 article published in JoVE Immunology and Infection Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System Marieke de Korte1,2, Armand Keating1,2,4, Chen Wang1,3 1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 2Krembil Research Institute, Toronto Western Hospital, University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital, Sinai Health Systems, 4Medical Oncology and Hematology, Princess Margaret Cancer Centre This is a step-by-step guide for using a commercially available rotary cell culture system to culture lymphocytes in simulated microgravity using specialized disposable culture vessels. This culturing method may be applied to any suspension-type cell culture.
