University Health Network, Toronto General Hospital 3 articles published in JoVE Medicine Normothermic Ex Vivo Pancreas Perfusion for the Preservation of Pancreas Allografts before Transplantation Catherine Parmentier1, Samrat Ray1, Laura Mazilescu1,2, Masataka Kawamura1, Yuki Noguchi1, Emmanuel Nogueira1, Sujani Ganesh1, Bhranavi Arulratnam1, Sangeetha Kalimuthu3, Markus Selzner1, Trevor Reichman1 1Ajmera Transplant Program, University Health Network, Toronto General Hospital, 2Department of General, Visceral, and Transplantation Surgery, University Hospital Essen, 3Department of Pathology, University Health Network, Toronto General Hospital Normothermic ex vivo machine perfusion (NEVP) has scarcely been explored for the preservation of pancreas allografts. We present an innovative preservation technique for pancreas allografts before transplantation. Medicine Surgical Tips and Tricks for Performing Porcine Pancreas Transplantation Samrat Ray1, Catherine Parmentier1, Laura Mazilescu1,2, Masataka Kawamura1, Yuki Noguchi1, Emmanuel Nogueira1, Sujani Ganesh1, Bhranavi Arulratnam1, Markus Selzner1, Trevor Reichman1 1Ajmera Transplant Centre, University Health Network, Toronto general hospital, 2Department of general, visceral, and transplantation surgery, University Hospital Essen The video article summarizes the technique of pancreatectomy and pancreas allotransplantation in a porcine 3-day survival model with a step-by-step description of the method and emphasis on the surgical tips and tricks to deal with the precarious and delicate porcine visceral anatomy. Bioengineering Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation Aisha Adil1,2, Golnaz Karoubi1,3, Siba Haykal1,2,4 1Latner Thoracic Research Surgical Laboratories, University Health Network, Toronto General Hospital, 2Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, 3Institute of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 4Division of Plastic & Reconstructive Surgery, Department of Surgery, University of Toronto We describe the surgical technique and decellularization process for composite rat hindlimbs. Decellularization is conducted using low-concentration sodium dodecyl sulfate through an ex vivo machine perfusion system.
