National Autonomous University of Mexico 7 articles published in JoVE Medicine Evaluation of Changes in Hydration and Body Cell Mass with Bioelectrical Impedance Analysis after Exercise Program for Rheumatoid Arthritis Patients Mariel Lozada-Mellado1,2, José M. García-Morales3, Midori Ogata-Medel3, Juan A. Pineda-Juárez4, Lilia Castillo-Martínez2,3 1Faculty of Higher Studies Ignacio Zaragoza, National Autonomous University of Mexico, 2Master's and Doctoral Program in Medical, Dental and Health Sciences, National Autonomous University of Mexico, 3Clinical Nutrition Service, National Institute of Medical Sciences and Nutrition Salvador Zubirán, 4Research Coordination, National Medical Center November 20 This protocol assesses alterations in hydration and body cell mass status using bioelectrical impedance vectorial analysis following a dynamic exercise program designed for patients with rheumatoid arthritis. The dynamic exercise program itself is detailed, highlighting its components focused on cardiovascular capacity, strength, and coordination. The protocol details steps, instruments, and limitations. Medicine Clinical Application of Phase Angle and BIVA Z-Score Analyses in Patients Admitted to an Emergency Department with Acute Heart Failure Fernanda Bernal-Ceballos1,2, Lilia Castillo-Martínez3, Yunuen Reyes-Paz3, José Luis Villanueva-Juárez3, Thierry Hernández-Gilsoul4 1Master's and Doctoral Program in Medical, Dental, and Health Sciences, National Autonomous University of Mexico, 2Faculty of Higher Studies Ignacio Zaragoza, National Autonomous University of Mexico, 3Clinical Nutrition Service, National Institute of Medical Sciences and Nutrition Salvador Zubirán, 4Emergency Department, National Institute of Medical Sciences and Nutrition Salvador Zubirán In this protocol, we explain how to obtain and interpret phase angle values and bioelectrical impedance vectorial analysis (BIVA) Z-score obtained by bioelectrical impedance in patients with acute heart failure admitted to the Emergency Department and their clinical applicability as a predictive marker for the prognosis of a 90-day event. Neuroscience How to Obtain Reliable Visual Event-related Potentials in Newborns Lourdes Cubero-Rego1, Josefina Ricardo-Garcell1, Thalía Harmony1, María Corsi-Cabrera1,2 1Research Unit in Neurodevelopment, Institute of Neurobiology, National Autonomous University of Mexico, 2Sleep Laboratory, Faculty of Psychology, National Autonomous University of Mexico Several important points for obtaining high-quality reliable visual evoked potentials (VEPs) in newborns while minimizing variability and the risk of misleading prognoses are presented. Chemistry Grafting Multiwalled Carbon Nanotubes with Polystyrene to Enable Self-Assembly and Anisotropic Patchiness Josué Arenas-García1, Martha V. Escárcega-Bobadilla2, Gustavo A. Zelada-Guillén1 1Department of Analytical Chemistry, School of Chemistry, National Autonomous University of Mexico, 2Department of Organic Chemistry, School of Chemistry, National Autonomous University of Mexico A procedure for the synthesis of polystyrene-grafted multiwalled carbon nanotubes using successive chemical modification steps to selectively introduce the polymer chains to the sidewalls and their self-assembly via anisotropic patchiness is presented. Behavior fMRI Mapping of Brain Activity Associated with the Vocal Production of Consonant and Dissonant Intervals Nadia González-García1, Pablo L. Rendón2 1 The neural correlates of listening to consonant and dissonant intervals have been widely studied, but the neural mechanisms associated with production of consonant and dissonant intervals are less well known. In this article, behavioral tests and fMRI are combined with interval identification and singing tasks to describe these mechanisms. Immunology and Infection In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis Nohemi Salinas-Jazmín1,2, Edith González-González1, Luz X. Vásquez-Bochm3, Sonia M. Pérez-Tapia4,5, Marco A. Velasco-Velázquez6 1Unit for Development and Research in Bioprocesses Unit (UDIBI), National School of Biological Sciences, National Polytechnic Institute (IPN), University of Mexico (UNAM), 2School of Chemistry, National Autonomous University of Mexico (UNAM), 3Graduate Program in Chemical Sciences, National Autonomous University of Mexico (UNAM), 4Unit for Development Research and Medical Innovation in Biotechnology (UDIMEB), National School of Biological Sciences, National Polytechnic Institute (IPN), 5Department of Immunology, National Scool of Biological Sciences, National Polytechnic Institute (IPN), 6Department of Pharmacology and Unit of Translational Biomedicine (CMN 20 de noviembre), School of Medicine, National Autonomous University of Mexico (UNAM) This protocol describes the in vitro comparison of two key functional characteristics of rituximab: target binding and complement-dependent cytotoxicity (CDC) induction. The methods were employed for a side-to-side comparison between reference rituximab and a rituximab biosimilar. These assays can be employed during biosimilar development or as a quality control in their production. Chemistry HKUST-1 as a Heterogeneous Catalyst for the Synthesis of Vanillin Rebeca Yépez1, Juan F. Illescas1, Paulina Gijón1, Manuel Sánchez-Sánchez2, Eduardo González-Zamora3, Rosa Santillan4, J. Raziel Álvarez5, Ilich A. Ibarra5, Julia Aguilar-Pliego1 1Autonomous Metropolitan University-Azcapotzalco, 2Institute of Catalysis and Petroleum Chemistry, ICP-CSIC, 3Department of Chemistry, Autonomous Metropolitan University-Iztapalapa, 4Department of Chemistry, Center of Investigation and Superior Studies (IPN), 5Research Institute of Material, National Autonomous University of Mexico The conversion of trans-ferulic acid to vanillin was achieved by heterogeneous catalysis. HKUST-1 was employed in this synthesis and the essential step in the catalytic process was the generation of unsaturated metal sites. Thus, when the catalyst was activated under vacuum, full vanillin conversion (yield of 95%) was obtained.
