Centro de Investigacion Cientifica de Yucatan 1 article published in JoVE Biochemistry Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions Roberto Pech-Kú1, J. Armando Muñoz-Sánchez1, Miriam Monforte-González1, Felipe Vázquez-Flota1, Beatriz A. Rodas-Junco2, S.M. Teresa Hernández-Sotomayor1 1Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, 2CONACYT, Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingeniería, Universidad Autónoma de Yucatán This protocol describes an efficient methodology for the extraction and quantification of caffeine in cell suspensions of C. arabica L. and an experimental process for evaluating the enzymatic activity of caffeine synthase with the expression level of the gene that encodes this enzyme.
