Nacional Institute of Neurology and Neurosurgery 1 article published in JoVE Medicine Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells Dulce Avila-Rodríguez1, Karina Paisano-Cerón1, Irene Valdovinos- Ramírez1, Carmen Solano-Agama1, Alma Ortiz-Plata2, María E. Mendoza-Garrido1 1Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies (CINVESTAV-IPN), 2Department of Experimental Pathology, Nacional Institute of Neurology and Neurosurgery Three-dimensional culture in alginate beads, due to its simplicity and reproducibility, was chosen to maintain the pituitary adenoma cells. The procedures included an initial enzymatic digestion and mechanical dissociation of the tumor tissue, and the subsequent cell suspension was encapsulated in alginate beads.
