Hospital General de Mexico “Dr. Eduardo Liceaga” 3 articles published in JoVE Neuroscience Rapid and Efficient Enrichment of Mouse Spinal Cord Microglia Claudia I Gutiérrez-Román1,2, María E Meléndez Camargo2, Cuauhtémoc C García Rojas3, Miguel Jimenez Olvera4, Sandra H Gutiérrez Román5, Oscar Medina-Contreras1 1 Microglia are regarded as some of the most versatile cells in the body, capable of morphological and functional adaptation. Their heterogeneity and multifunctionality enable the maintenance of brain homeostasis, while also being linked to various neurological pathologies. Here, a technique for purifying spinal cord microglia is described. Medicine Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy Benjamín León-Mancilla1,2, Moisés Martínez-Castillo1, Zaira Medina-Avila1, Armando Pérez-Torres3, Jorge Garcia-Loya2, Ana Alfaro-Cruz4, Cristina Piña-Barba5, Gabriela Gutierrez-Reyes1 1Liver, Pancreas and Motility Laboratory; Unit of Experimental Medicine; School of Medicine, Universidad Nacional Autónoma de Mexico (UNAM), 2Department of Surgery, School of Medicine, Universidad Nacional Autónoma de México (UNAM), 3Department of Cells and Tissue Biology, School of Medicine, Universidad Nacional Autónoma de México (UNAM), 4Department of Pathology, Hospital General de Mexico, 5Materials Research Institute, Universidad Nacional Autónoma de México (UNAM) Liver diseases are induced by many causes that promote fibrosis or cirrhosis. Transplantation is the only option for recovering health. However, given the scarcity of transplantable organs, alternatives must be explored. Our research proposes the implantation of collagen scaffolds in liver tissue from an animal model. Medicine A Model of Experimental Steatosis In Vitro: Hepatocyte Cell Culture in Lipid Overload-Conditioned Medium Adriana Campos-Espinosa1, Carolina Guzmán1 1Laboratorio de Hígado, Páncreas y Motilidad, Unidad de Medicina Experimental, Facultad de Medicina-UNAM, Hospital General de México “Dr. Eduardo Liceaga” This protocol is intended to be a tool to study steatosis and the molecular, biochemical, cellular changes produced by the over exposure of hepatocytes to lipids in vitro.
