Instituto de Oftalmologia Fundacion Conde de Valenciana IAP 1 article published in JoVE Medicine Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature Luis Haro-Morlett*1, Fatima Sofia Magaña-Guerrero*1, Beatriz Buentello Volante1, Yonathan Garfias1,2 1Instituto de Oftalmologia Fundacion Conde de Valenciana IAP, 2Biochemistry Department, Medicine Faculty, Universidad Nacional Autónoma de Mexico The present protocol describes the extraction of lumican from the amniotic membrane (AM) and their storing conditions as AM extract (AME) at -20 °C, 4 °C, and room temperature (RT) for 6, 12, 20, and 32 days to quantify its proteins and lumican concentration.
