Phoenix VA Health Care System 2 articles published in JoVE Neuroscience Simultaneous Cryosectioning of Multiple Rodent Brains Tabitha R.F. Green1,2,3, J. Bryce Ortiz1, Jordan L. Harrison5, Jonathan Lifshitz1,3,4, Rachel K. Rowe1,3,4 1Department of Child Health, University of Arizona College of Medicine, 2Department of Biological Sciences, University of Bath, 3BARROW Neurological Institute at Phoenix Children's Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences, University of Arizona College of Medicine Here, we present a protocol to freeze and section brain tissue from multiple animals as a timesaving alternative to processing single brains. This reduces staining variability during immunohistochemistry and reduces time cryosectioning and imaging. Neuroscience Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons Megan N. Evilsizor1,2, Helen F. Ray-Jones1,3, Jonathan Lifshitz1,2,4,5, Jenna Ziebell1,2 1Department of Child Health, University of Arizona College of Medicine - Phoenix, 2 This introductory level protocol describes the reagents, equipment, and techniques required to complete immunohistochemical staining of rodent brains, using markers for microglia and neuronal elements as an example.
