Maricopa Medical Center and St Josephs Hospital and Medical Center 1 article published in JoVE Medicine Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens Lee J. Pribyl1, Kathleen A. Coughlin1, Thanasak Sueblinvong1, Kristin Shields2, Yoshie Iizuka1,3, Levi S. Downs1,3, Rahel G. Ghebre1,3, Martina Bazzaro1,3 1Department of Obstetrics, Gynecology, and Women's Heath, University of Minnesota, 2Department of Obstetrics and Gynecology, Maricopa Medical Center and St Josephs Hospital and Medical Center, 3Masonic Cancer Center, University of Minnesota This study describes a detailed method for isolation and characterization of primary ovarian cancer cells from solid clinical specimens. Ovarian cancer clinical specimens are subjected to enzymatic digestion to obtain viable, fibroblast-free epithelial ovarian cancer (EOC) cells highly suitable for downstream applications.
