Arizona Alzheimer's Consortium 1 article published in JoVE Genetics Identification of Circular RNAs using RNA Sequencing Shobana Sekar1,2, Philipp Geiger1,2, Lori Cuyugan1,2, Annalee Boyle1,2, Geidy Serrano2,3, Thomas G. Beach2,3, Winnie S. Liang1,2 1Translational Genomic Research Institute, 2 Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented here.
