BioLegend 1 article published in JoVE Immunology and Infection Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform Jason S. Lehmann1, Amy Zhao1, Binggang Sun1, Weiping Jiang1, Shaoquan Ji1 1BioLegend This protocol describes the quantification of multiple cytokine targets simultaneously in tissue culture supernatants collected from stimulated mouse splenocytes using multiplex bead based immunoassay platform and a flow cytometer.