UC San Diego School of Medicine 4 articles published in JoVE Developmental Biology Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease Sandra L. Leibel1,2,3, Rachael N. McVicar2,3, Alicia M. Winquist2,3, Evan Y. Snyder1,2,3 1Department of Pediatrics, University of California, San Diego School of Medicine, 2Sanford Consortium for Regenerative Medicine, 3Sanford Burnham Prebys Medical Discovery Institute The article describes step wise directed differentiation of induced pluripotent stem cells to three-dimensional whole lung organoids containing both proximal and distal epithelial lung cells along with mesenchyme. Neuroscience Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion Richard B. McCosh1, Michael J. Kreisman1, Kellie M. Breen1 1Department of Reproductive Medicine, University of California, San Diego School of Medicine Here we present a tail-tip blood sampling protocol for frequent sample collection in unrestrained mice. This method is useful for assessing patterns of pulsatile luteinizing hormone secretion and could be adapted for analysis of other circulating factors. Immunology and Infection A Murine Model of Group B Streptococcus Vaginal Colonization Kathryn A. Patras1, Kelly S. Doran1,2 1Department of Pediatrics, Division of Host-Microbe Systems & Therapeutics, University of California San Diego School of Medicine, 2Department of Biology and Center for Microbial Sciences, San Diego State University The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies. Neuroscience Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis Sylvia Neumann1, George E. Campbell*1, Lukasz Szpankowski*2,3, Lawrence S.B. Goldstein2,4, Sandra E. Encalada1 1Department of Molecular and Experimental Medicine, Dorris Neuroscience Center, The Scripps Research Institute, 2Department of Cellular and Molecular Medicine, University of California San Diego, 3Department of Bioengineering, University of California San Diego, 4Department of Neurosciences, University of California San Diego School of Medicine Intracellular transport of cargoes, such as vesicles or organelles, is carried out by molecular motor proteins that track on polarized microtubules. This protocol describes the correlation of the directionality of transport of individual cargo particles moving inside neurons, to the relative amount and type of associated motor proteins.
