Blood Systems Research Institute 1 article published in JoVE Biology Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry Heather Inglis1, Philip Norris1,2,3, Ali Danesh1,3 1Blood Systems Research Institute, 2Department of Medicine, University of California, San Francisco, 3Department of Laboratory Medicine, University of California, San Francisco Many different methods exist for the measurement of extracellular vesicles (EVs) using flow cytometry (FCM). Several aspects should be considered when determining the most appropriate method to use. Two protocols for measuring EVs are presented, using either individual detection or a bead-based approach.