Archer Daniels Midland 1 article published in JoVE Biochemistry Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle Heidi A. Rossow1, Gabriela Acetoze2, John Champagne1, Jon J. Ramsey3 1Veterinary Medicine Teaching and Research Center, University of California Davis, 2Archer Daniels Midland, 3Department of Molecular Biosciences, University of California Davis Here, we share methods for measuring mitochondrial oxygen consumption, a defining concept of nutritional energetics, and proton leak, the primary cause of inefficiency in mitochondrial generation of ATP. These results can account for 30% of the energy lost in nutrient utilization to help evaluate mitochondrial function.
