Neurophotometrics Ltd. 1 article published in JoVE Neuroscience Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals Ekaterina Martianova1, Sage Aronson2,3, Christophe D. Proulx1 1CERVO Brain Research Center, Department of Psychiatry and Neurosciences, Université Laval, 2Center for Neural Circuits and Behavior, Department of Neuroscience and Section of Neurobiology, Division of Biology, University of California at San Diego, 3Neurophotometrics Ltd. This protocol details how to implement and perform multi-fiber photometry recordings, how to correct for calcium-independent artifacts, and important considerations for dual-color photometry imaging.