Saint Mary's College of California 1 article published in JoVE Neuroscience Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis Megan Holt1, Brandon Adams1, Vidya Chandrasekaran1 1 This paper describes the isolation and culturing of embryonic rat sympathetic neurons from the superior cervical ganglia. It also provides detailed protocols for immunocytochemical staining and for preparing neuronal extracts for mass spectrometric analysis.
