Denver VA Medical Center 2 articles published in JoVE Medicine An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse Marilee J. Wick1,2, Julie W. Harral1,2, Zoe L. Loomis1,2, Edward C. Dempsey1,2,3 1Cardiovascular Pulmonary Research Laboratory, University of Colorado Denver, 2Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Denver, 3Denver VA Medical Center In this article, an economical, optimized, and simple protocol is described which uses the Evans blue dye method for assessing plasma extravasation in the organs of FVBN mice that can be adapted for use in other strains, species, and other organs or tissues. Neuroscience Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture Ron Bouchard1,2, Thomas Chong1,2, Subbiah Pugazhenthi1,2 1Section of Endocrinology, Denver VA Medical Center, 2Department of Medicine, University of Colorado Denver School of Medicine Human neuroprogenitor cells (NPCs) were expanded under proliferating conditions. NPCs were differentiated into neuron-rich cultures in the presence of a combination of neurotrophins. Neuronal markers were detected by immunofluorescence staining. To isolate a pure population of neurons, NPCs were differentiated on PEN membrane slides and laser capture microdissection was performed.
