University of Colorado, Aurora 1 article published in JoVE Medicine Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing Teresa T. Liu*1,2, Allison C. Rodgers*3, Tristan M. Nicholson4, Jill A. Macoska5,6, Paul C. Marker6,7, Chad M. Vezina6,8, Dale E. Bjorling6,8, Alejandro Roldan-Alzate2,9,10, Diego Hernando10,11, Granville L. Lloyd12, Timothy A. Hacker3, William A. Ricke1,6 1Department of Urology, University of Wisconsin-Madison, 2K12 Kure, University of Wisconsin-Madison, 3Cardiovascular Research Center, Department of Medicine, University of Wisconsin-Madison, 4Department of Urology, University of Washington, 5University of Massachusetts Boston, 6U54 George M. O'Brien Center, University of Wisconsin-Madison, 7College of Pharmacy, University of Wisconsin-Madison, 8School of Veterinary Medicine, University of Wisconsin-Madison, 9Department of Mechanical Engineering, University of Wisconsin-Madison, 10Department of Radiology, University of Wisconsin-Madison, 11Department of Medical Physics, University of Wisconsin-Madison, 12Rocky Mountain Regional VA Medical Center, University of Colorado We describe the use of high frequency ultrasound with contrast imaging as a method to measure bladder volume, bladder wall thickness, urine velocity, void volume, void duration, and urethral diameter. This strategy can be used to assess voiding dysfunction and treatment efficacy in various mouse models of lower urinary tract dysfunction (LUTD).