Sanford Burnham Prebys Medical Discovery Institute at Lake Nona 1 article published in JoVE Developmental Biology Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation Zoltan Simandi*1, Attila Horvath*2, Peter Nagy1, Laszlo Nagy1,2,3 1Sanford-Burnham-Prebys Medical Discovery Institute at Lake Nona, 2Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, 3MTA-DE “Lendulet” Immunogenomics Research Group, University of Debrecen In this work we provide an experimental workflow of how active enhancers can be identified and experimentally validated.