Florida Institute of Technology 1 article published in JoVE Environment Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans Nicole L. Golden1, Rosemary N. Plagens1, Karen S. Kim Guisbert1, Eric Guisbert1 1Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology Here, standardized protocols are presented to assess induction of the heat shock response (HSR) in Caenorhabditis elegans using RT-qPCR at the molecular level, fluorescent reporters at the cellular level, and thermorecovery at the organismal level.