University of Florida Institute of Food and Agricultural Sciences 1 article published in JoVE Biology A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates James C. Fulton*1, Matthew A. Cullen*1, Kristin Beckham1, Tatiana Sanchez2, Zhuxuan Xu1, Preston Stern1, Gary Vallad3, Geoffrey Meru4,5, Cecilia McGregor6, Nicholas S. Dufault1 1Department of Plant Pathology, University of Florida, 2University of Florida Institute of Food and Agricultural Sciences, 3Gulf Coast Research and Education Center, University of Florida, 4Horticultural Sciences Department, University of Florida, 5Tropical Research and Education Center, University of Florida, 6Department of Horticulture, University of Georgia Managing Fusarium wilt of watermelon requires knowledge of the pathogen races present. Here, we describe the root-dip, infested kernel seeding, and modified tray-dip inoculation methods to demonstrate their efficacy in race-typing of the pathogenic fungus Fusarium oxysporum f. sp niveum (Fon).
